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In vivo imaging of mucosal CD4+ T cells using single photon emission computed tomography in a murine model of colitis

Identifieur interne : 006517 ( Main/Repository ); précédent : 006516; suivant : 006518

In vivo imaging of mucosal CD4+ T cells using single photon emission computed tomography in a murine model of colitis

Auteurs : RBID : Pascal:08-0112512

Descripteurs français

Pascal (Inist)
- Souris, Muqueuse, Lymphocyte T, Modèle animal, Méthode immunologique, Colite, Antigène CD4.

English descriptors

KwdEn :
- Animal model, Colitis, Immunological method, Mouse, Mucosa, T-Lymphocyte.

Abstract

Immune responses that occur in the context of human infectious and inflammatory diseases are usually studied by sampling cells from peripheral blood, from biopsies, or by end-point harvests at necropsy. These approaches are likely to yield information that is incomplete and/or non-representative. Here, we report the development and validation of a non-invasive method to localize and to quantitate the disposition of specific subpopulations of cells in vivo. In a murine model of dextran sulfate sodium (DSS)-induced colitis, CD4⁺ T cells were visualized in the colon by single photon emission computed tomography (SPECT-CT) after injection of monoclonal, non-depleting, indium-111 (¹¹¹In) labeled anti-CD4⁺ antibodies. The SPECT-CT colon uptake ratio (CUR) was found to correlate (p<0.01) with the number of total CD4⁺T cells and with standard measures of pathology (colon length, cell counts, and histopathologic evidence of apoptosis, edema, and cellular infiltrates) as assessed by direct examination of diseased colon. Each of these parameters, including the SPECT-CT signal uptake, increased as a function of DSS dose (p<0.05). We conclude that CT-SPECT imaging using an ¹¹¹In-labeled anti-CD4⁺ antibody is reflective of traditional parameters of pathology in this experimental model of murine colitis. This approach should be readily applicable to the imaging of discrete cell subpopulations in non-human primates and in humans, thus augmenting our understanding of infectious diseases and inflammation in vivo.

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Pascal:08-0112512

Le document en format XML

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 T cells using single photon emission computed tomography in a murine model of colitis</title>
<author><name sortKey="Kanwar, Bittoo" uniqKey="Kanwar B">Bittoo Kanwar</name>
<affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Department of Medicine, Division of Experimental Medicine, The University of California, San Francisco</s1>
<s2>San Francisco, CA</s2>
<s3>USA</s3>
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<country>États-Unis</country>
<placeName><settlement type="city">San Francisco</settlement>
<region type="state">Californie</region>
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<affiliation wicri:level="3"><inist:fA14 i1="02"><s1>Department of Pediatrics, Division of Gastroenterology, Hepatology, and Nutrition, The University of California, San Francisco</s1>
<s2>San Francisco, CA</s2>
<s3>USA</s3>
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<author><name>DONG WEI GAO</name>
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<author><name sortKey="Hwang, Andrew B" uniqKey="Hwang A">Andrew B. Hwang</name>
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<front><div type="abstract" xml:lang="en">Immune responses that occur in the context of human infectious and inflammatory diseases are usually studied by sampling cells from peripheral blood, from biopsies, or by end-point harvests at necropsy. These approaches are likely to yield information that is incomplete and/or non-representative. Here, we report the development and validation of a non-invasive method to localize and to quantitate the disposition of specific subpopulations of cells in vivo. In a murine model of dextran sulfate sodium (DSS)-induced colitis, CD4<sup>+</sup>
 T cells were visualized in the colon by single photon emission computed tomography (SPECT-CT) after injection of monoclonal, non-depleting, indium-111 (<sup>111</sup>
In) labeled anti-CD4<sup>+</sup>
 antibodies. The SPECT-CT colon uptake ratio (CUR) was found to correlate (p<0.01) with the number of total CD4<sup>+</sup>
T cells and with standard measures of pathology (colon length, cell counts, and histopathologic evidence of apoptosis, edema, and cellular infiltrates) as assessed by direct examination of diseased colon. Each of these parameters, including the SPECT-CT signal uptake, increased as a function of DSS dose (p<0.05). We conclude that CT-SPECT imaging using an <sup>111</sup>
In-labeled anti-CD4<sup>+</sup>
 antibody is reflective of traditional parameters of pathology in this experimental model of murine colitis. This approach should be readily applicable to the imaging of discrete cell subpopulations in non-human primates and in humans, thus augmenting our understanding of infectious diseases and inflammation in vivo.</div>
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In vivo imaging of mucosal CD4+ T cells using single photon emission computed tomography in a murine model of colitis

In vivo imaging of mucosal CD4+ T cells using single photon emission computed tomography in a murine model of colitis

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri